What is FSC and SSC in flow?
Answer. In flow cytometry, the light scattered by cells is measured by two optical detectors: forward scatter (FSC) that detects scatter along the path of the laser, and side scatter (SSC) which measures scatter at a ninety-degree angle relative to the laser.
What does FSC-A mean?
forward scatter area
forward scatter area (FSC-A) density plot can be used to exclude doublets as shown in Figure 3 below. A side scatter height (SSC-H) vs side scatter area (SSC-A) plot can also be used. This type of gating can be more sensitive for doublet exclusion as the FSC detector is not usually a photomultiplier tube (PMT).
What does FSC mean in flow cytometry?
forward scatter
The light scatter is measured by two optical detectors. One detector measures scatter along the path of the laser (1). This parameter is referred to as forward scatter (FSC). The other detector measures scatter at a ninety degree angle relative to the laser (1). This parameter is called side scatter (SSC).
Why is the FSC important?
FSC provide the highest standard of protection for endangered species and forests, providing a safe and secure future for our planet.
What does an FSC label do?
The FSC Recycled label indicates that the FSC has verified the use of 100% post-consumer or post-consumer reclaimed, recycled materials. This is impactful for two reasons: the first being that products from consumers are not put into landfills.
What do my flow cytometry results mean?
What Do the Results Mean? Flow cytometry can provide information that is used to diagnose, stage and monitor blood cancers. It can also be used to test for minimal residual disease (MRD), the number of cancer cells remaining in the body after treatment.
How do you analyze flow cytometry?
Flow cytometers utilize properties of fluid dynamics to send cells one at a time through a laser. The optics and computer systems then track the photon emission from excited cells and analyze both the light that scatters past (forward scatter; FSC) and the light that scatters perpendicularly (side scatter; SSC).
What cells are CD45-negative?
The CD45 antigen is normally universally expressed in variable intensities on all leukocytes. Usually, this marker is used to gate out non-nucleated cells. In this case, however, the CD45-negative follicular lymphoma cells were almost overlooked using the normal gating strategy.
What cells are CD45 expressed on?
CD45 (lymphocyte common antigen) is a receptor-linked protein tyrosine phosphatase that is expressed on all leucocytes, and which plays a crucial role in the function of these cells.
What is PE in flow cytometry?
Phycoerythrin, also known as R-PE, is typically used for flow cytometry, microarrays, ELISA, and other applications that require high sensitivity but not photostability.
What are FSC standards?
FSC certification ensures that products come from responsibly managed forests that provide environmental, social and economic benefits. The FSC Principles and Criteria provide a foundation for all forest management standards globally, including the FSC US National Standard (v1.
Why is FSC good for the environment?
It protects the environment. FSC eliminates deforestation, forest degradation and illegal logging in certified areas. Moreover, it is the organization’s endeavor to grow the market for sustainable forest products and to highlight the dangers of poor forestry practice.
What are the FSC standards?
In short, a product can be certified by the Forest Stewardship Council (FSC), which means it meets the “gold standard” ethical production. The wood is harvested from forests that are responsibly managed, socially beneficial, environmentally conscious, and economically viable.
Why is FSC important?
Raise standards: FSC certification acts as a catalyst for improving standards in forest management. It brings economic benefits by opening up new markets and helps ensure social benefits to workers and local people, and facilitates environmental benefits for biodiversity and ecosystems.