How does the Luminex assay work?
Using a Luminex instrument, beads are excited by one laser to determine the bead region and corresponding assigned analyte. Another laser determines the magnitude of the PE-derived signal, which is proportional to the amount of analyte bound. Multiple readings are taken at each bead region, ensuring robust detection.
How do you analyze data Luminex?
To analyze and perform quality control on Luminex data, a lab might follow these steps:
- Set up an assay project.
- Create an assay design.
- Import data and enter metadata.
- Explore results.
- Perform within-run quality control.
- Perform cross-run quality control.
- Share quality-controlled data with collaborators.
Is Luminex an Elisa?
Key differences between ELISA and LUMINEX assays are the use of different target capture systems and different reporter systems. LUMINEX assays capture targets onto spherical beads in suspension while ELISAs generally rely on flat surfaces in multi-well plates to capture targets.
What is Luminex screening?
A Principle of the Luminex assay to detect HLA antibodies. Each specific bead impregnated with two fluorophores is coated with HLA from cell lines and platelets or recom-binant HLA. In case the test serum contains antibodies directed against the specific HLA it will bind to the appropriate bead.
Is Luminex more sensitive than ELISA?
Thus, the two assays are comparable for specificity and accuracy, and the Luminex assay is more sensitive than the ELISA.
What is Luminex used for?
Luminex Platform Luminex multiplex assays use xMAP bead-based technology to simultaneously detect and quantitate multiple secreted proteins including cytokines, chemokines, and growth factors. This multiplex technology can also be used to measure gene expression utilizing the Invitrogen QuantiGene Plex assays.
Is Luminex quantitative?
Highly-quantitative multiplex analysis of cytokines, chemokines, growth factors, metabolites, etc. in low amount of sample.
What is Luminex blood test?
Luminex xMAP (Multi-Analyte Profiling) Clinical Information. Test is used for the detection and identification of antibodies directed to donor-specific HLA class I antigens by single-antigen bead-based flow cytometry.
How long does a Luminex assay take?
about 30 mins to 1 hour
Generally, each Luminex assay takes about 30 mins to 1 hour to analyze a 96 well plate on the platform.
What is Luminex technology?
Luminex xMap technology is a bead-based multiplexed immunoassay system in a microplate format. The system can simultaneously detect many targets in a single sample — up to 500, depending on system design. The microspheres used in the Luminex system have different spectral addresses (color codes).
What is Luminex instrument?
Multiplexing instrumentation for the measurement of proteins and RNA (gene expression) The Luminex family of systems includes options for various multiplexing capabilities, throughputs, and read times. Compare our systems below to determine which Luminex instrument is right for your laboratory.
What is MFI Luminex?
The objective of this study was to explore the near-accurate mean fluorescence intensity (MFI) cut-off values detected on Luminex platform predicting the strength of cell-based crossmatch results.
What is HLA Luminex?
The Luminex-based human leukocyte antigen (HLA) antibody screening technology is widespread used in laboratories affiliated to kidney transplantation programs and enables both screening (i.e. the definition of positive or negative antibody status) and antibody identification with high sensitivity and specificity.
How does the Luminex Magpix work?
A magnet in the MAGPIX analyzer captures and holds the magnetic beads in a monolayer, while two spectrally distinct light-emitting diodes (LEDs) illuminate the beads. One LED identifies the analyte that is being detected and, the second LED determines the magnitude of the PE-derived signal.
What is flex map?
The FLEXMAP 3D is Luminex’s most advanced and versatile multiplexing platform. Fast read time, compatibility with 96- and 384-well plates, and ease of integration with front-end automation systems make the FLEXMAP 3D the platform of choice for high-throughput applications.
What is Magpix?
MAGPIX is a compact fluorescent-based detection system, capable of simultaneously measuring up to 50 targets of interest in a single microtiter well. Based on Luminex’s proven xMAP® Technology, the MAGPIX system uses color-coded magnetic microspheres as the substrate on which assays are performed.
Is Luminex more sensitive than Elisa?
What is a Luminex machine?
The Luminex 200 sets the standard for multiplexing. This instrument is a popular choice for laboratories needing greater flexibility. Multiplexing: Up to 100 analytes per sample volume (80 on MagPlex microspheres) Dynamic Range (Typical): >3.5 logs.
How much does a Luminex cost?
Discovery Price $31.95/sample (single analysis / includes sample filtration)